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Triplex addressability as a basis for functional DNA nanostructures.

机译：三重寻址能力作为功能性DNA纳米结构的基础。

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摘要

Here, we present the formation of a fully addressable DNA nanostructure that shows the potential to be exploited as, for example, an information storage device based on pH-driven triplex strand formation or nanoscale circuits based on electron transfer. The nanostructure is composed of two adjacent hexagonal unit cells (analogous to naphthalene) in which each of the eleven edges has a unique double-stranded DNA sequence, constructed using novel three-way oligonucleotides. This allows each ten base-pair side, just 3.4 nm in length, to be assigned a specific address according to its sequence. Such constructs are therefore an ideal precursor to a nonrepetitive two-dimensional grid on which the "addresses" are located at a precise and known position. Triplex recognition of these addresses could function as a simple yet efficient means of information storage and retrieval. Future applications that may be envisaged include nanoscale circuits as well as subnanometer precision in nanoparticle templating. Characterization of these precursor nanostructures and their reversible targeting by triplex strand formation is shown here using gel electrophoresis, atomic force microscopy, and fluorescence resonance energy transfer (FRET) measurements. The durability of the system to repeated cycling of pH switching is also confirmed by the FRET studies.
机译：在这里，我们介绍了一个完全可寻址的DNA纳米结构的形成，该结构显示了被开发为例如基于pH驱动的三链链形成的信息存储设备或基于电子转移的纳米级电路的潜力。纳米结构由两个相邻的六边形单位细胞（类似于萘）组成，其中的十一个边缘均具有独特的双链DNA序列，该序列是使用新型三向寡核苷酸构建的。这样就可以根据序列顺序为长度仅为3.4 nm的每十个碱基对侧分配一个特定的地址。因此，这种构造是“地址”位于精确且已知位置的非重复二维网格的理想先驱。这些地址的三重识别可以用作信息存储和检索的简单而有效的手段。可以设想的未来应用包括纳米级电路以及纳米粒子模板化中的亚纳米精度。使用凝胶电泳，原子力显微镜和荧光共振能量转移（FRET）测量结果显示了这些前体纳米结构的特征及其通过三链形成形成的可逆靶向性。 FRET研究还证实了系统对pH转换重复循环的耐久性。

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